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Rosengren J Biol Chem Such carriers enable the compounds according to the present invention to be formulated as tablets, pills, capsules, dragees, liquids, gels, syrups, slurries, suspensions and the like, for oral ingestion by a subject to be treated. T Cell Proliferation and Cytokine Production. The transformed prokaryotic hosts can be grown in fermentors and cultured according to techniques known in the art to achieve optimal cell growth. Recipient rats may either be treated with a control or the component i. Vaccination with the cyclotide resulted in a reduction in the incidence and severity of EAE.

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The cells were transferred into a well plate, centrifuged 5 min, RPM, 20° C.

US9453052B2 - Cyclotides as immunosuppressive agents - Google Patents

Said host cell may be a prokaryotic or eukaryotic cell; see supra. After annexin V staining, propidium iodide solution PI; eBioscience was added and the cells were incubated in the dark, followed by a flow cytometric analysis to determine the amount of apoptosis and necrosis. The extracts were concentrated on a roto-evaporator and were lyophilized. It will also be acknowledged by the ones skilled in the art that one or several of the amino acids forming the cyclotide to be employed according to the present invention may be modified. The present invention is further described by reference to the following non-limiting figures and examples. For transmucosal and transpulmonal administration, penetrants appropriate to the barrier to be permeated are used in the formulation.


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